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小鼠鑰孔蟲戚血藍(lán)蛋白酶免試劑盒,(KLH)ELISA檢測試劑盒 ,英文名:KLH ELISA Kit ,英文縮寫:KLH ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠鑰孔蟲戚血藍(lán)蛋白ELISA檢測試劑盒,KLH 檢測試劑盒, KLH 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠鑰孔蟲戚血藍(lán)蛋白酶免試劑盒,(KLH)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
β-位淀粉樣前體蛋白裂解酶1(βACE1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Site APP Cleaving Enzyme 1 (bACE1)48T/96TY12735Rattus norvegicus (Rat)
早老素1(PSEN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Presenilin 1 (PSEN1)48T/96TY12736Rattus norvegicus (Rat)
血管非炎性蛋白1(VNN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vanin 1 (VNN1)48T/96TY12737Homo sapiens (Human)
鈣/鈣調(diào)蛋白依賴性蛋白激酶Ⅱα(CAMK2α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a)48T/96TY12738Rattus norvegicus (Rat)
拓?fù)洚悩?gòu)酶Ⅱβ(TOP2β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Topoisomerase II Beta (TOP2b)48T/96TY12739Homo sapiens (Human)
再生胰島衍生蛋白3γ(REG3γ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 3 Gamma (REG3g)48T/96TY12740Homo sapiens (Human)
異連蛋白β(DTNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dystrobrevin Beta (DTNb)48T/96TY12741Homo sapiens (Human)
脊椎蛋白1(SPON1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Spondin 1 (SPON1)48T/96TY12742Homo sapiens (Human)
互聯(lián)蛋白神經(jīng)元中間絲蛋白α(INα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Internexin Neuronal Intermediate Filament Protein Alpha (INa)48T/96TY12743Homo sapiens (Human)
Lin-28同源物A(LIN28A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lin-28 Homolog A (LIN28A)48T/96TY12744Mus musculus (Mouse)
組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY12745Canis familiaris; Canine (Dog)
受磷蛋白(PLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholamban (PLN)48T/96TY12746Mus musculus (Mouse)
無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)48T/96TY12747Homo sapiens (Human)
再生胰島衍生蛋白3γ(REG3γ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 3 Gamma (REG3g)48T/96TY12748Mus musculus (Mouse)
蘭尼定受體1(RYR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ryanodine Receptor 1, Skeletal (RYR1)48T/96TY12749Mus musculus (Mouse)
核因子I/B(NFIB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor I/B (NFIB)48T/96TY12750Mus musculus (Mouse)
R-脊椎蛋白1(RSPO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for R-Spondin 1 (RSPO1)48T/96TY12751Homo sapiens (Human)
神經(jīng)顆粒素(NRGN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurogranin (NRGN)48T/96TY12752Homo sapiens (Human)
橋粒聯(lián)結(jié)蛋白(AHNAK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Desmoyokin (AHNAK)48T/96TY12753Homo sapiens (Human)
前列腺蛋白類脂肪酶B(LIPB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipophilin B, Prostatein Like (LIPB)48T/96TY12754Homo sapiens (Human)
β-位淀粉樣前體蛋白裂解酶1(βACE1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Site APP Cleaving Enzyme 1 (bACE1)48T/96TY12755Rattus norvegicus (Rat)
早老素1(PSEN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Presenilin 1 (PSEN1)48T/96TY12756Rattus norvegicus (Rat)
血管非炎性蛋白1(VNN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vanin 1 (VNN1)48T/96TY12757Homo sapiens (Human)
鈣/鈣調(diào)蛋白依賴性蛋白激酶Ⅱα(CAMK2α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a)48T/96TY12758Rattus norvegicus (Rat)
拓?fù)洚悩?gòu)酶Ⅱβ(TOP2β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Topoisomerase II Beta (TOP2b)48T/96TY12759Homo sapiens (Human)
再生胰島衍生蛋白3γ(REG3γ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 3 Gamma (REG3g)48T/96TY12760Homo sapiens (Human)
異連蛋白β(DTNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dystrobrevin Beta (DTNb)48T/96TY12761Homo sapiens (Human)
脊椎蛋白1(SPON1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Spondin 1 (SPON1)48T/96TY12762Homo sapiens (Human)
互聯(lián)蛋白神經(jīng)元中間絲蛋白α(INα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Internexin Neuronal Intermediate Filament Protein Alpha (INa)48T/96TY12763Homo sapiens (Human)
Lin-28同源物A(LIN28A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lin-28 Homolog A (LIN28A)48T/96TY12764Mus musculus (Mouse)
組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY12765Canis familiaris; Canine (Dog)
受磷蛋白(PLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholamban (PLN)48T/96TY12766Mus musculus (Mouse)
無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)48T/96TY12767Homo sapiens (Human)
再生胰島衍生蛋白3γ(REG3γ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 3 Gamma (REG3g)48T/96TY12768Mus musculus (Mouse)
蘭尼定受體1(RYR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ryanodine Receptor 1, Skeletal (RYR1)48T/96TY12769Mus musculus (Mouse)
核因子I/B(NFIB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor I/B (NFIB)48T/96TY12770Mus musculus (Mouse)
R-脊椎蛋白1(RSPO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for R-Spondin 1 (RSPO1)48T/96TY12771Homo sapiens (Human)
神經(jīng)顆粒素(NRGN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurogranin (NRGN)48T/96TY12772Homo sapiens (Human)
橋粒聯(lián)結(jié)蛋白(AHNAK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Desmoyokin (AHNAK)48T/96TY12773Homo sapiens (Human)
肌鈣蛋白T(TNT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin T (TNT)48T/96TY12774Canis familiaris; Canine (Dog)
分泌跨膜蛋白1(SECTM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secreted And Transmembrane Protein 1 (SECTM1)48T/96TY12775Homo sapiens (Human)
肥大細(xì)胞類糜蛋白酶1(CMA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chymase 1, Mast Cell (CMA1)48T/96TY12776Mus musculus (Mouse)
無翅型MMTV整合位點家族成員3(WNT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 3 (WNT3)48T/96TY12777Homo sapiens (Human)
無翅型MMTV整合位點家族成員2(WNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 2 (WNT2)48T/96TY12778Homo sapiens (Human)
無翅型MMTV整合位點家族成員1(WNT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 1 (WNT1)48T/96TY12779Homo sapiens (Human)
序列相似家族5成員C(FAM5C)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Family With Sequence Similarity 5, Member C (FAM5C)48T/96TY12780Homo sapiens (Human)
白三烯A4水解酶(LTA4H)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukotriene A4 Hydrolase (LTA4H)48T/96TY12781Homo sapiens (Human)
芳香烴受體核轉(zhuǎn)位因子樣蛋白(ARNTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL)48T/96TY12782Rattus norvegicus (Rat)
核受體亞家族1D組成員2(NR1D2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2)48T/96TY12783Rattus norvegicus (Rat)
粘蛋白4(MUC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 4 (MUC4)48T/96TY12784Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。