服務(wù)熱線
021-54479081
021-54461587
產(chǎn)品展示PRODUCTS
本產(chǎn)品僅用于科研,不用于臨床
人血吸蟲酶免試劑盒,(schistosoma)ELISA檢測試劑盒,英文名:schistosoma ELISA Kit,英文縮寫:schistosoma ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人血吸蟲ELISA檢測試劑盒,schistosoma 檢測試劑盒,schistosoma 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人血吸蟲酶免試劑盒,(schistosoma)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
可溶性半乳糖凝集素3結(jié)合蛋白(LGALS3BP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP)48T/96TY09985Homo sapiens (Human)
微小染色體維持缺陷蛋白2(MCM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Minichromosome Maintenance Deficient 2 (MCM2)48T/96TY09986Homo sapiens (Human)
S100鈣結(jié)合蛋白A6(S100A6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A6 (S100A6)48T/96TY09987Homo sapiens (Human)
糖蛋白M6A(GPM6A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycoprotein M6A (GPM6A)48T/96TY09988Homo sapiens (Human)
細(xì)胞分裂周期因子23(CDC23)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cell Division Cycle Protein 23 (CDC23)48T/96TY09989Homo sapiens (Human)
角質(zhì)轉(zhuǎn)谷氨酰胺酶(TGM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transglutaminase 1, Keratinocyte (TGM1)48T/96TY09990Homo sapiens (Human)
髓細(xì)胞核分化抗原(MNDA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myeloid Cell Nuclear Differentiation Antigen (MNDA)48T/96TY09991Homo sapiens (Human)
干擾素調(diào)節(jié)因子8(IRF8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Regulatory Factor 8 (IRF8)48T/96TY09992Homo sapiens (Human)
涎福林蛋白(SPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sialophorin (SPN)48T/96TY09993Homo sapiens (Human)
類粘蛋白2(ORM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Orosomucoid 2 (ORM2)48T/96TY09994Homo sapiens (Human)
磷脂爬行酶1(PLSCR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipid Scramblase 1 (PLSCR1)48T/96TY09995Homo sapiens (Human)
CD7分子(CD7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 7 (CD7)48T/96TY09996Homo sapiens (Human)
腦啡肽酶(NEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neprilysin (NEP)48T/96TY09997Homo sapiens (Human)
受體相互作用絲氨酸蘇氨酸激酶2(RIPK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Receptor Interacting Serine Threonine Kinase 2 (RIPK2)48T/96TY09998Homo sapiens (Human)
Rho家族GTP酶1(RND1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Rho Family GTPase 1 (RND1)48T/96TY09999Homo sapiens (Human)
S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)48T/96TY10000Homo sapiens (Human)
S100鈣結(jié)合蛋白A9(S100A9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A9 (S100A9)48T/96TY10001Homo sapiens (Human)
血清淀粉樣蛋白A2(SAA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serum Amyloid A2 (SAA2)48T/96TY10002Homo sapiens (Human)
信號傳導(dǎo)轉(zhuǎn)錄激活因子2(STAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Signal Transducer And Activator Of Transcription 2 (STAT2)48T/96TY10003Homo sapiens (Human)
CD5分子(CD5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 5 (CD5)48T/96TY10004Homo sapiens (Human)
激肽釋放酶原(PK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prekallikrein (PK)48T/96TY10005Homo sapiens (Human)
白介素21(IL21)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 21 (IL21)48T/96TY10006Rattus norvegicus (Rat)
轉(zhuǎn)化生長因子β刺激蛋白22(TSC22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22)48T/96TY10007Rattus norvegicus (Rat)
歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Homing Associated Cell Adhesion Molecule (HCAM)48T/96TY10008Homo sapiens (Human)
高分子量激肽原(HMWK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for High Molecular Weight Kininogen (HMWK)48T/96TY10009Homo sapiens (Human)
粘蛋白2(MUC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 2 (MUC2)48T/96TY10010Mus musculus (Mouse)
粘蛋白7(MUC7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 7, Secreted (MUC7)48T/96TY10011Homo sapiens (Human)
棕櫚酰化膜蛋白2(MPP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Membrane Protein, Palmitoylated 2 (MPP2)48T/96TY10012Homo sapiens (Human)
乙醇脫氫酶1(ADH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)48T/96TY10013Homo sapiens (Human)
硒蛋白P1(SEPP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Selenoprotein P1, Plasma (SEPP1)48T/96TY10014Homo sapiens (Human)
維生素D結(jié)合蛋白(DBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vitamin D Binding Protein (DBP)48T/96TY10015Homo sapiens (Human)
簇集蛋白(CLU)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Clusterin (CLU)48T/96TY10016Rattus norvegicus (Rat)
尿嘧啶核苷磷酸化酶1(UPP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uridine Phosphorylase 1 (UPP1)48T/96TY10017Homo sapiens (Human)
肝腎骨堿性磷酸酶(ALPL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)48T/96TY10018Rattus norvegicus (Rat)
β-位淀粉樣前體蛋白裂解酶2(βACE2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Site APP Cleaving Enzyme 2 (bACE2)48T/96TY10019Homo sapiens (Human)
β-位淀粉樣前體蛋白裂解酶2(βACE2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Site APP Cleaving Enzyme 2 (bACE2)48T/96TY10020Rattus norvegicus (Rat)
纖溶酶原(Plg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen (Plg)48T/96TY10021Rattus norvegicus (Rat)
白介素6受體(IL6R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 6 Receptor (IL6R)48T/96TY10022Rattus norvegicus (Rat)
甲狀旁腺激素(PTH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY10023Chicken (Gallus)
多功能蛋白聚糖(VS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Versican (VS)48T/96TY10024Homo sapiens (Human)
血管內(nèi)皮生長因子受體1(VEGFR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 1 (VEGFR1)48T/96TY10025Homo sapiens (Human)
V-Jun肉瘤病毒癌基因同源物(JUN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)48T/96TY10026Rattus norvegicus (Rat)
V-Jun肉瘤病毒癌基因同源物(JUN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)48T/96TY10027Mus musculus (Mouse)
V-Fos-FBJ骨肉瘤病毒癌基因同源物(FOS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)48T/96TY10028Rattus norvegicus (Rat)
V-Fos-FBJ骨肉瘤病毒癌基因同源物(FOS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)48T/96TY10029Mus musculus (Mouse)
胰腺再生蛋白Ⅳ(REG4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 4 (REG4)48T/96TY10030Homo sapiens (Human)
肌鈣蛋白T(TNT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin T (TNT)48T/96TY10031Mus musculus (Mouse)
鞘氨醇(Sph)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sphingosine (Sph)48T/96TY10032General
角蛋白17(KRT17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 17 (KRT17)48T/96TY10033Homo sapiens (Human)
核因子κB(NFκB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor Kappa B (NFkB)48T/96TY10034Chicken (Gallus)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。